Trials are special missions in All Star Tower Defense that requires players to be of a certain level to play. Many people seem to misspell Trials and say "Trails". When completed, each trial rewards the player with a 5-star unit. When completed in Extreme Mode, Trials will also give an exclusive Orb in addition to a 5-star unit.
|Trial||Level required||Possible enemies||Trial boss||Anime||Reward|
|Lvl 25+||May be any currently released unit in the game, except for event characters, all 6 stars, EXP characters, and Christmas Gifts.||Godus||Dragon Ball|
|Lvl 35+||Heart Tank||Yoshaga Kiryu||JoJo's Bizarre Adventure|
|Lvl 45+||mrflimflam string clones.||MrFlimFlam||One Piece|